Research Articles

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  Research Article | Spotlight

  The Endoplasmic Reticulum Cargo Receptor SURF4 Facilitates Efficient Erythropoietin Secretion

  Erythropoietin (EPO) stimulates erythroid differentiation and maturation. Though the transcriptional regulation of EPO has been well studied, the molecular determinants of EPO secretion remain unknown. Here, we generated a HEK293T reporter cell line that provides a quantifiable and selectable readout of intracellular EPO levels and performed a genome-scale CRISPR screen that identified SURF4 as an important mediator of EPO secretion....

  Zesen Lin, Richard King, Vi Tang, Greggory Myers, Ginette Balbin-Cuesta, Ann Friedman, Beth McGee, Karl Desch, Ayse Bilge Ozel, David Siemieniak, Pavan Reddy, Brian Emmer, Rami Khoriaty
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  Research Article

  Loss of Mitochondrial Localization of Human FANCG Causes Defective FANCJ Helicase

  Fanconi anemia (FA) is a unique DNA damage repair pathway. To date, 22 genes have been identified that are associated with the FA pathway. A defect in any of those genes causes genomic instability, and the patients bearing the mutation become susceptible to cancer. In our earlier work, we identified that Fanconi anemia protein G (FANCG) protects the mitochondria from oxidative stress. In this report, we have identified eight patients...

  Jagadeesh Chandra Bose K, Bishwajit Singh Kapoor, Kamal Mandal, Shubhrima Ghosh, Raveendra B. Mokhamatam, Sunil K. Manna, Sudit S. Mukhopadhyay